Aria 8.0 Bedienungsanleitung

University of Massachusetts Medical School Flow Cytometry Core Facility BD FACSAria II Self-Sorting Training Program Prepared by Marc Barnard Table

EXAMPLE SET-UP: 5-peak sort exercise Set-up for a “2-way” sort of peaks 1 and 5 Sort these populations Workspace Sort layout Browser Inspector Acqu

Example Sort report Page 11

Example Post-sort Peak 1 “Purity check” Post-sort Peak 5 “Purity check” Page 12

Example sort setup for a cell line Sorting for RFP positive cells Page 13

Task Bullet List with “Clog Fix” by Susanne Pechhold ● Turn on computer ● Turn on instrument ● Log on to Windows and FACSDiva, start Coherent Connecti

FACS Aria Sort Check List by Glenn Paradise Did you: 1) Put the FACS Aria cover down. 2) Turn the stream on if it is off. Give it time to stabilize. 3

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Introduction Please drop by the main lab (S5-322) or call the core at (6-3276) to discuss the training program. LSR training and use is a pre-requisi

FACSAria training program overview: 1) A 2 hour introduction to the FACSAria II with a practice bead sort. This will include stopping the stream,

Starting up the system During the work week the core will have the Aria ready for use. You will need to log on, start the stream and examine the brea

Cleaning and Shutting down the system You must clean the instrument as detailed below at the end of your sort. Always Filter 10% Bleach and Coulter C

Typically , 2nd drop 10-20, 3rd drop 5-10, 4th drop 0-5 Optimizing the side streams 1) Click the Voltage button in the side stream window to turn on

Optimized stream Aiming the streams Once the center and side streams are optimized, and the sweet spot is on aim the streams for a 2-way (2 populatio

Setting the drop delay with Accudrop Beads The Flow Core has made an “Accudrop delay” experiment. Please use this for setting drop delay. This is on

Performing a sort using 5-peak beads See the next page for example setup 1) Create a new experiment, use the new experiment button in the toolbar 2)