Aria 8.0 Betriebsanweisung PDF herunterladen (Seite 118)

118 BD FACSAria II User’s Guide

Starting the Stream

The system can take anywhere from 20 seconds to several minutes to reach the

correct pressure and turn on the stream, depending on the state of the system and

the sort setup selected.

1 Start the stream.

a Click the Sorting button ( ) on the Workspace toolbar to display the

Breakoff and Side Stream windows.

b Click the Stream button ( ) in the Breakoff window to turn on the

stream.

2 Open the sort block door and check the stream.

The stream should flow smoothly from the nozzle into the center of the

waste aspirator.

If the stream is flowing but is unsteady, check for bubbles in the flow cell. If

you see bubbles, turn off the stream, wait for 10 seconds, and turn on the

stream again.

If you see any dripping or spraying, or the stream image appears abnormal,

turn off the stream and see Troubleshooting the Stream on page 234.

3 Close the sort block door.

NOTE If you encounter bubbles in the sheath fluid stream at the

130 micron sort setting, perform a sample line backflush for approximately

1 minute. Select Cytometer > Cleaning Modes > Sample Line Backflush.

To prevent th is, the sheath tank should be depressurized while not in use to

prevent air from dissolving into the sheath solution.

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Aria 8.0 Betriebsanweisung Seite 118