Aria 8.0 Betriebsanweisung PDF herunterladen (Seite 172)

172 BD FACSAria II User’s Guide

5 Click Cancel at the Confirm dialog.

There is no need to collect the beads. When the drawer is closed, the beads

are sorted to waste.

6 Adjust the micrometer dial (see Figure 1-20 on page 38) to obtain the

brightest bead spot on the center stream.

7 Click the Auto Delay button in the Side Stream window.

A dialog similar to Figure 5-5 on page 173 opens. The graph in the left

pane of the dialog represents brightness of the stream (Y axis) versus drop

delay (X axis). Typically the graph should have a flat portion, with several

small peaks in either direction (up or down) or one prominent peak.

NOTE If the original drop delay value was close to the appropriate value

prior to starting auto delay, then you may not get a graph displayed

because few data points were required to determine the optimal drop delay.

8 Select Start Run in the Auto Drop Delay dialog.

9 Monitor the Auto Drop Delay dialog for progress. A message appears

when the process is completed.

NOTE If the sort is stopped during algorithm execution (either by user

action or because the system detected a failure), the run will be stopped

with an appropriate message. Start the sort again, verify that the stream is

stable and the sort is not pausing, and re-run the auto drop delay.

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Aria 8.0 Betriebsanweisung Seite 172