Aria 8.0 Betriebsanweisung PDF herunterladen (Seite 50)

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50 BD FACSAria II User’s Guide

Signal Detection

From the cuvette flow cell, scattered and fluorescent light is collected by the

fluorescence objective lens. The lens collects and focuses fluorescent light emitted

at each of the laser focal points onto individual collection fibers. These fibers

transfer the emitted light to the individual detector arrays.

The following sections describes how laser light is detected and translated into

signals that can be displayed in a plot.

Detector Arrays

There are two types of detector arrays in the BD FACSAria II flow cytometer: an

octagon and trigons. The octagon detects SSC and up to seven fluorescence

signals excited by the 488-nm (blue) laser. Th e t ri go ns detect fluorescence signals

excited by the 633-nm (red) and 405-nm (violet) lasers, respectively. Each trigon

can detect up to three fluorescence channels. (An optical upgrade is required to

fill all detection channels.)

Figure 2-6 Octagon and trigon detector arrays

Each detector array houses dichroic and bandpass filters, which steer and filter

the emitted light, and photomultiplier tubes (PMTs), which detect light signals.

blue-laser signalred-laser signalviolet-laser signal

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