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70 BD FACSAria II User’s Guide

Yield Mask

The Yield Mask setting defines how close to the edge of the drop, in 1/32-drop

increments, a particle of interest can be located before sorting an additional drop.

Half of each Yield Mask setting defines an equal area at each end of the drop.

For example, when the Yield Mask is set to 16 and an event is within 8/32 from

the beginning of a drop, the previous (leading) drop will be sorted. If an event is

within 8/32 from the end of a drop, the following (trailing) drop will be sorted.

See Figure 2-18.

Figure 2-18 Target particle within a Yield Mask of 16

If the Yield Mask were set to 8 for the same target particle, the target particle

would fall outside of the Yield Mask; therefore, no additional drops would be

sorted. See Figure 2-19.

Figure 2-19 Target particle outside a Yield Mask of 8

When the Yield Mask is set to zero, only one drop (the drop containing the target

particle) will be deflected. When the mask is set to 32, two drops will always be

deflected. Yield Masks between 0–32 will sort either one or two drops.

Yield Masks cannot be used in conjunction with Phase Masks. Therefore, when

the Yield Mask is greater than zero, the Phase Mask automatically reverts to

zero.

trailing drop: sorted drop being interrogated leading drop: not sorted

target particle

Yield Mask

trailing drop: not sorted drop being interrogated leading drop: not sorted

Yield Mask

target particle

Yield Mask

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Aria 8.0 Betriebsanweisung Seite 70